Phytochemical screening and antidiabetic effect of extracts of the seeds of Citrullus lanatus in alloxan-induced diabetic albino mice
نویسنده
چکیده
Article history: Received on: 07/09/2014 Revised on: 23/10/2014 Accepted on: 14/12/2014 Available online: 28/03/2015 Diabetes mellitus is a major and current epidemic disease of the human race implicated with numerous clinical manifestations. A number of protein-rich seeds such as that of Citrullus lanatus (watermelon) are commonly used in traditional medicine with increasing acclaimed efficacy against diabetes mellitus. In this study the effects of petroleum ether and ethanol extracts of the seeds of Citrullus lanatus on blood glucose levels in alloxan-induced diabetes in mice have been investigated. Hyperglycemia was induced by the injection of 150 mg/kg (i.p.) of alloxan monohydrate freshly dissolved in physiological saline. Doses (150, 200 and 250 mg/kg) per os, of the extracts were separately administered to a group of five diabetic mice in the study. The activity was compared with reference standard glibenclamide (2 mg/kg, p.o.) and negative control of physiological saline. Treatment of alloxan-induced diabetic mice with the crude extracts of C. lanatus seeds brought down the raised blood glucose levels significantly (P < 0.05) in a dose-dependent manner. The ethanol extract was found to have more antidiabetic effect than the petroleum ether extract. Phytochemical screening of the seed extracts of Citrullus lanatus indicated the presence of steroids, alkaloids, flavonoids, terpenoids, and saponins in both the ethanol and petroleum ether extracts. In addition, anthraquinones, tannins and reducing sugar were detected in the ethanol extract.
منابع مشابه
Antidiabetic activity and phytochemical screening of extracts of the leaves of Ajuga remota Benth on alloxan-induced diabetic mice
BACKGROUND Ajuga remota Benth is traditionally used in Ethiopia for the management of diabetes mellitus. Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic effect and phytochemical screening of the aqueous and 70% ethanol extracts on alloxan-induced diabetic mice. METHODS After acute toxicity test, the Swiss albino mice were induc...
متن کاملPhytochemical Sreening and Antidiabetic Antioxidant Effect of Ecbolium Ligustrinum Flowers Extracts
The antidiabetic and antioxidant activity of Ecbolium ligustrinum (family:Acanthaceae) was investigated in alloxan induced diabetic albino rats. A comparison was made between both plant extracts and a known antidiabetic drug Glibenclamide (5mg/kg body weight). The dried flowers of Ecbolium ligustinum was subjected to extraction by continuous hot extraction method using hydroalcohol and chlorofo...
متن کاملPhytochemical and Antioxidant Activities of Berberis integerrima and Berberis vulgaris and Pharmacological Effects of the more Active Species on Alloxan-Induced Diabetic Rats
Background: Medicinal plants with biologically active components such as antioxidant and antidiabetic are useful sources of novel therapeutics. In Iran, traditionally believed that the aqueous extract of berberry fruit (Berberis integerima F.) improved health, especially in people with diabetes. Objective: In this study, in vitro phytochemical and antioxidant activities of B. integerrima and...
متن کاملAntidiabetic and Antihyperlipidemic Effects of Methanolic Extract of Citrullus Lanatus Seeds in Rats
Objective: Aim of the present study is to evaluate the antidiabetic and antihyperlipidemic effect of methanolic extract of Citrullus lanatus seeds (MECL) in streptozotocin (STZ) induced diabetic rats. Methods: Diabetes was induced by a single dose of STZ (65 mg/kg) in citrate buffer, while the normal control group was given the vehicle only. After three days of induction of diabetes, the diabet...
متن کاملIsolation, Fractionation and Evaluation of the Anti-Inflammatory Properties of Citrullus lanatus Thumb
The main objective of this experiment was to investigate the antiinflammatory activity of Citrullus lanatus methanol extract (ME) and its fractions in adult male albino rats. Citrullus lanatus has been traditionally used for various disease and disorders. The phytochemical evaluation of methanolic seeds extract showed the presence of carbohydrate, alkaloids, steroids, saponins, flavonoids, tann...
متن کامل